Rabbit Anti-Human VEGFR-1/Flt-1 (Peptide), soluble
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| Cat-Nr. | 102-PA21 |
| Size | 200 µg |
| Price | 290 € |
| Category | Polyclonal Antibody |
| Clone Nr. | Rabbit IgG |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Preparation | Produced from sera of rabbits immunised with a peptide of the C-terminal end of native human soluble VEGFR-1/Flt-1 (GEHCNKKAVFSRISKFKSTR NDSTTQSNVKH). Anti-human soluble VEGFR-1/Flt-1 was purified by affinity chromatography with immobilized Protein A. |
| Antigen | Peptide: GEHCNKKAVFSRISKFKSTR NDSTTQSNVKH. |
| Application | WB |
| Synonyms | soluble vascular endothelial growth factor receptor-1; soluble FLT1; soluble FLT; soluble VEGF receptor 1 |
| Description | Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes, dendritic cells and on trophoblast cells. The flt-1 gene was first described in 1990. The receptor contains seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular splited tyrosine kinase domain. Compared to VEGFR-2 the Flt-1 receptor has a higher affinity for VEGF but a weaker signaling activity. VEGFR-1 thus leads not to proliferation of endothelial cells, but mediates signals for differentiation. Interestingly a naturally occuring soluble variant of VEGFR-1 (sVEGFR-1) was found in HUVEC supernatants in 1996, which is generated by alternative splicing of the flt-1 mRNA. |
| Uniprot ID | P17948 |
| Protein RefSeq | NP_001153392 |
| mRNA RefSeq | NM_001159920 |
Figures
Immunofluorescence staining (green) of two neighboring sections of a human vein (V), located near a hemangioma. The antibody against the soluble VEGFR-1/Flt-1 marked single cells (arrows) within the media and adventitia of the vein. The antibody against the membrane-bound VEGFR-1/Flt-1 marked single cells (arrows) and the endothelium (arrowhead) of the vein. Cell nuclei are stained with Dapi (blue).
Provided by Prof. J. Wilting, Göttingen, Germany.
Western Analysis of anti-human esFlt-1. Samples were loaded in 10% SDS-polyacrylamide gel under reducing conditions. Left panel: monoclonal antibody against Flt-1; Right panel: polyclonal antibody (peptide) against the unique C-terminal end of esFlt-1.
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