Rabbit Anti-Human CEACAM-1 (dN)
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| Cat-Nr. | 102-PA102S |
| Size | 100 µg |
| Price | 185 € |
| Category | Polyclonal Antibody |
| Clone Nr. | Rabbit IgG |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Antigen | Recombinant human sCEA-1 (ΔN) |
| Application | E, WB, FC |
| Synonyms | CEACAM1; BGP; BGP1; BGPI, CD66a |
| Description | Carcinoembryonic antigen (CEA)-related cell adhesion molecule 1 (CEACAM1; also BGP) is a 160 kDa member of the CEACAM branch of the CEA gene family of the immunoglobulin superfamily (1-3). It is one of seven human CEACAM subfamily genes that are essentially divided equally between type I trans-membrane proteins (CEACAM1, 3-4) and GPI-linked molecules (CEACAM5-8). There is no CEACAM2 in human. The gene for human CEACAM1 codes for a 526 amino acid (aa) type I transmembrane protein that contains a 34 aa signal sequence, a 394 aa extracellular domain (ECD), a 24 aa transmembrane segment, and a 74aa cytoplasmic region (4, 5). The ECD contains one N-terminal V-type followed by three C2-type Ig-like domains. It shows considerable glycosylation (1). The cytoplasmic region shows one ITIM motif and a calmodulin binding site (1-3). There are three soluble and seven transmembrane isoforms, with variations occurring in both the ECD and cytoplasmic region. All ten alternate splice forms contain the V-type Ig-like domain (aa’s 35-142). The three soluble forms also contain the first two C2-type Ig like domains (aa’s 145 317), with differences coming in the third C2-type Ig- like domain (6). The seven transmembrane isoforms are highly divergent. Full-length mouse and rat CEACAM1 are approximately 57% aa identical to human CEACAM1; in the V-type Ig-like domain, they are 58% and 56% aa identical, respectively. The full-length molecule is found on neutrophils, bile duct epithelium, activated NK cells, colonic columnar epithelium and endothelium. |
| Uniprot ID | P13688 |
| Protein RefSeq | NP_001192273.1 |
| mRNA RefSeq | NM_001205344 |
Figures
Western blot analysis of total lysate of different CEACAM proteins expressing cell lines with the polyclonal rabbit anti-human CEACAM1 antibody [Cat# 102-PA102, #dN]. There is a very slight cross-reactivity with human CEACAM5 visible.
The experiments were performed by PD Dr. Bernhard Singer, Institute of Anatomy, Medical Faculty, University Duisburg-Essen, Germany.
Immunohistochemistry (IHC) staining of CEACAM1 in human prostate tissue using the polyclonal rabbit anti-human CEACAM1 antibody [Cat# 102-PA102, #dN] with DAB staining.
The experiments were performed by PD Dr. Bernhard Singer, Institute of Anatomy, Medical Faculty, University Duisburg-Essen, Germany.
Immunohistochemistry (IHC) staining of CEACAM1 in human prostate tissue using the polyclonal rabbit anti-human CEACAM1 antibody [Cat# 102-PA102, #dN] with DAB staining.
The experiments were performed by PD Dr. Bernhard Singer, Institute of Anatomy, Medical Faculty, University Duisburg-Essen, Germany.
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