Rabbit Anti-Human Amphiregulin
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| Cat-Nr. | 102-PA09S |
| Size | 100 µg |
| Price | 175 € |
| Category | Polyclonal Antibody |
| Clone Nr. | Rabbit IgG |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Antigen | recombinant human Amphiregulin |
| Application | WB, IF |
| Synonyms | AREG; AR; SDGF; AREGB; CRDGF |
| Description | Amphiregulin is an EGF related growth factor that signals through the EGF/TGF-a receptor, and stimulates growth of keratinocytes, epithelial cells and some fibroblasts. Amphiregulin also inhibits the growth of certain carcinoma cell lines. Synthesized as a transmembrane protein, Amphiregulin’s extracellular domain is proteolytically processed to release the mature protein. There are 6 conserved cysteine residues, which form 3 intramolecular disulfide bonds essential for biological activity. Recombinant human Amphiregulin is a 11.3 kDa glycoprotein consisting of 98 amino acid residues. |
| Uniprot ID | P15514 |
| Protein RefSeq | NP_001648.1 |
| mRNA RefSeq | NM_001657.2 |
Figures
Immunofluorescence staining of human Amphiregulin in a mixture of primary human endothelial cells (HDLEC, HUVEC) and primary normal human dermal fibroblast (NHDF) with a polyclonal rabbit anti-human Amphiregulin antibody [Cat# 102-PA09]. Conjugated secondary antibody: donkey anti-rabbit ALEXA Flour 488 (1:600) [Dianova].
Western Blot Analysis of recombinant human Amphiregulin [Cat# 200-011] using a rabbit anti-human Amphiregulin polyclonal antibody.
Immunofluorescence staining of human Amphiregulin in primary normal human dermal fibroblasts (NHDF) and primary human dermal lymphatic endothelial cells (HDLEC) with a polyclonal rabbit anti-human Amphiregulin antibody [Cat# 102-PA09]. Conjugated secondary antibody: donkey anti-rabbit ALEXA Flour 488 (1:600) [Dianova].
Immunofluorescence staining (cryo-sections) of vasa vasorum of a human lymphatic collector. (fixed 15 min in 4% PFA) with anti-human Amphiregulin (5µg/ml) [Cat# 102-PA09] and counter staining of nuclei with Dapi. [400X]
The experiment was performed by the research group of Prof. Dr. J. Wilting and Dr. K. Buttler, University Göttingen, Germany.
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