Rabbit Anti-Human CD31/PECAM-1
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| Cat-Nr. | 102-PA07S |
| Size | 100 µg |
| Price | 185 € |
| Category | Polyclonal Antibody |
| Clone Nr. | Rabbit IgG |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Antigen | Recombinant human soluble CD31 |
| Application | WB, IF, FC |
| Synonyms | PECAM-1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM |
| Description | PECAM is transmembrane glycoprotein that belongs to the Ig-related superfamily of adhesion molecules. It is highly expressed at endothelial cell junctions, and also expressed in platelets and in most leukocyte sub-types. The primary function of PECAM-1 is the mediation of leukocyte-endothelial cell adhesion and signal transduction. PECAM-1 has been implicated in the pathogenesis of various inflammation related disorders, including thrombosis, multiple sclerosis (MS), and rheumatoid arthritis. The human PECAM-1 gene codes for a 738 amino acid transmembrane glycoprotein containing a 118 amino acid cytoplasmic domain, a 19 amino acid transmembrane domain, and a 574 amino acid extracellular domain. Recombinant human PECAM-1 is a 572 amino acid glycoprotein comprising the extracellular domain of PECAM-1. Monomeric glycosylated PECAM-1 migrates at an apparent molecular weight of approximately 80.0-95.0 kDa by SDS-PAGE analysis under reducing conditions. |
| Uniprot ID | P16284 |
| Protein RefSeq | NP_000433.4 |
| mRNA RefSeq | NM_000442.4 |
Figures
Immunofluorescence staining of human CD31 in a mixture of primary human endothelial cells (HDLEC, HUVEC) and primary normal human dermal firbroblast (NHDF) with a polyclonal rabbit anti-human CD31 antibody [Cat# 102-PA07]. Conjugated secondary antibody: donkey anti-rabbit ALEXA Flour 488 (1:600) [Dianova].
Immunofluorescence staining (cryo-sections) of vasa vasorum of a human lymphatic collector. (fixed 15 min in 4% PFA) with anti-human CD31 (5µg/ml) [Cat# 102-PA07] and counter staining of nuclei with Dapi. [400X]
The experiment was performed by the research group of Prof. Dr. J. Wilting and Dr. K. Buttler, University Göttingen, Germany.
Western Blot Analysis of recombinant human sCD31 [Cat# S01-071] using a rabbit anti-human CD31 polyclonal antibody.
Immunofluorescence staining of human CD31 in primary human dermal lymphatic endothelial cells (HDLEC) and primary human umbilical vein endothelial cells (HUVEC) with a polyclonal rabbit anti-human CD31 antibody [Cat# 102-PA07]. Conjugated secondary antibody: donkey anti-rabbit ALEXA Flour 488 (1:600) [Dianova].
FACS analysis of CD31 expression in human primary umbilical vein endothelial cells.
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