Description / anti-human TIE-1 monoclonal antibody
TIE-1 (tyrosine kinase with Ig and EGF homology domains 1) and TIE-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis.
More Information
| Size | 100 µg |
|---|---|
| Source | Mouse |
| Isotype | IgG1 |
| Biological Activity | ELISA: Use at 1-15 µg/ml; Western blotting: Use at 1-2 µg/ml |
| Clone Nr. | (#8C9) |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% |
| Preparation | The monoclonal antibody was produced with the help of BALB/c mice using recombinant human soluble extracellular TIE-1 as the immunizing antigen. Mouse IgG1 antibody (#8C9) from hybridomas was purified from cell culture supernatant by Protein G chromatogra |
| Antigen | recombinant human soluble TIE-1 |
| Application | ELISA, WB |
| Synonyms | TIE1; TIE; JTK14 |
| Uniprot ID | P35590 |
| Protein RefSeq | NP_005415 |
| mRNA RefSeq | NM_005424 |