Description / human IL-2 protein
IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes. Recombinant human IL-2 (modified at the C-terminal end) is a 15.5 kDa protein, containing 156 amino acid residues including one intrachain disulfide bond.
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| Size | 10 µg |
|---|---|
| Source | E. coli |
| Biological Activity | The ED50 as determined by the dose-dependent stimulation of proliferation of murine D10G4.1 cells is in the range of 0.1-0.5 ng/ml. |
| N Terminal Sequence | APTSSSTK and MAPTSSST |
| Purity Confirmation | > 98% by SDS-PAGE |
| Length [aa] | 156 |
| Molecular Weight | 15.5 kDa |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | 50mM acetic acid |
| Protein Sequence | MAPTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNRWITFCQSIISTLHYKDPAANKARKEAELAAATAEQ |
| Reconstitution | The lyophilized IL-2 should be reconstituted in 50mM acetic acid to a concentration not less than 100µg/ml. This solution can be diluted into other buffered solutions or stored at -20°C for future use. |
| Stability and Storage | The lyophilized IL-2, though stable at room temperature, is best stored desiccated below 0°C. Reconstituted IL-2 should be stored in workingaliquots at -20°C. Avoid repeated freeze-thaw cycles. |
| Synonyms | IL2; IL-2; TCGF; lymphokine |
| Uniprot ID | P60568 |
| Protein RefSeq | NP_000577 |
| mRNA RefSeq | NM_000586 |