Description / human VCAM-1 protein
VCAM is a 110 kDa cell surface integral membrane glycoprotein that belongs to the Ig-related superfamily of adhesion molecules. The primary function of VCAM-1 is the mediation of leukocyte-endothelial cell adhesion and signal transduction. VCAM-1 may play a vital role in the development several diseases, including atherosclerosis and rheumatoid arthritis. The human VCAM-1 gene codes for a 715 amino acid transmembrane glycoprotein containing a 19 amino acid cytoplasmic domain, a 22 amino acid transmembrane domain, and a 674 amino acid extracellular domain. Recombinant human VCAM-1 is a 74.1 kDa glycoprotein comprising the extracellular domain (674 amino acid residues) of VCAM-1. Monomeric glycosylated VCAM-1 migrates at an apparent molecular weight of approximately 90.0 kDa by SDS-PAGE analysis under reducing conditions.
More Information
| Size | 10 µg |
|---|---|
| Source | HEK 293 cells |
| Biological Activity | Determined by its ability to support the adhesion of human U937 cells. The expected ED50 for this effect is 0.8-1.0 μg/ml. |
| Purity Confirmation | > 98% by SDS-PAGE & HPLC analyses |
| Length [aa] | 673 |
| Molecular Weight | 74 kDa |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Protein Sequence | FKIETTPESR YLAQIGDSVS LTCSTTGCES PFFSWRTQID SPLNGKVTNE GTTSTLTMNP VSFGNEHSYL CTATCESRKL EKGIQVEIYS FPKDPEIHLS GPLEAGKPIT VKCSVADVYP FDRLEIDLLK GDHLMKSQEF LEDADRKSLE TKSLEVTFTP VIEDIGKVLV CRAKLHIDEM DSVPTVRQAV KELQVYISPK NTVISVNPST KLQEGGSVTM TCSSEGLPAP EI |
| Synonyms | VCAM1; CD106; INCAM-100 |
| Uniprot ID | P19320 |
| Protein RefSeq | NP_542413.1 |
| mRNA RefSeq | NM_080682.2 |