Description / anti-human VCAM-1 monoclonal antibody
VCAM-1 is expressed on the surface of activated endothelial cells and macrophages. It binds to leukocyte integrins VLA-4 and alpha 4 beta 7 to promote leukocyte adhesion at sites of inflamed vasculature.
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| Size | 50 µg |
|---|---|
| Source | Mouse |
| Isotype | IgG1 |
| Biological Activity | WB: 1:100-1000; IHC (Formalin/Paraffin): 1:50-200 |
| Clone Nr. | (#6C12) |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml. |
| Stability and Storage | Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity. |
| Preparation | This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse) immunized with LPS activated HUVECs. IgG1 fraction of ascites fluid was purified by Protein A affinity chromatography. |
| Antigen | LPS activated HUVECs |
| Application | WB, IHC (P) |
| Synonyms | VCAM1; CD106; INCAM-100 |
| Uniprot ID | P19320 |
| Protein RefSeq | NP_542413.1 |
| mRNA RefSeq | NM_080682.2 |