Description / anti-mouse Podoplanin monoclonal antibody Biotin-conjugated
Podoplanin, also known as T1 alpha (T1α), is a mucin type transmembrane glycoprotein with extensive O-glycosylation. It is specifically expressed by lymphatic endothelial cells but not blood vascular endothelial cells. In addition, non-endothelial cells in numerous normal tissues also express the protein. Within the region used as the immunogen, mouse Podoplanin shares 73.5% and 29% amino acid sequence homology with rat and human Podoplanin, respectively.
More Information
| Size | 50 µg |
|---|---|
| Source | Hamster |
| Isotype | IgG |
| Biological Activity | Western Blot: use at 2-5 µg/ml. |
| Clone Nr. | (#RTD4E10) |
| Species Reactivity | Mouse |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% |
| Preparation | Monoclonal antibodies were produced by immunizing Syrian hamsters with a murine thymic stromal cell line. This antibody recognizes mouse Podoplanin. Hamster IgG antibody from hybridomas was purified from cell culture supernatant by Protein G chromatograph |
| Antigen | murine stromal cell line |
| Application | WB, IHC |
| Synonyms | Pdpn; T1a; Gp38; OTS-8; T1alpha; RANDAM-2; T1-alpha |
| Uniprot ID | Q62011 |
| Protein RefSeq | NP_034459.2 |
| mRNA RefSeq | NM_010329.2 |