Description / NP-1 (alpha-Defensin 1)
Defensins (α and β) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The α-defensins which include NP-1, NP-2 and NP-3, are distinguished from the β-defensins by the pairing of their three disulfide bonds. In addition to antimicrobial activity, NP-1 exhibits chemotactic activity on dendritic cells. NP-1 is expressed as the C-terminal portion of an inactive precursor protein, which also contains a 19 amino acid N-terminal signal sequence and a 45 amino acid polypeptide. NP-1 contains a six-cysteine motif that forms three intra-molecular disulfide bonds. Recombinant human NP-1 is a 3.4 kDa protein containing 30 amino acid residues.
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| Size | 5 µg |
|---|---|
| Source | E. coli |
| Biological Activity | Determined by its ability to induce adhesion of HUVECs. The expected ED50 for this effect is 1.5- 2.5 μg/ml. |
| Purity Confirmation | > 98% by SDS-PAGE & HPLC analyses |
| Length [aa] | 30 |
| Molecular Weight | 3.4 kDa |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Protein Sequence | ACYCRIPACI AGERRYGYCI YQGRLWAFCC |
| Reconstitution | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carri |
| Stability and Storage | The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C. |
| Synonyms | Neutrophil Peptide-1, alpha Defensin-1, HNP-1, Cryptdin |
| Uniprot ID | Q15818 |
| Protein RefSeq | NP_002513.2 |
| mRNA RefSeq | NM_002522.3 |