Description / mouse IL-1 beta protein
IL-1β is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1α and IL-1β binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1β is a secreted cytokine, IL-1α is predominantly a cell-associated cytokine. Recombinant murine IL-1β is a 17.5 kDa protein containing 153 amino acid residues.
More Information
| Size | 10 µg |
|---|---|
| Source | E. coli |
| Biological Activity | The ED50 as determined by the dose-dependent stimulation of murine D10S cells is ≤ 0.002 ng/ml, corresponding to a specific activity of ≥ 5 x 108 units/mg. |
| Purity Confirmation | > 98% by SDS-PAGE & HPLC analyses |
| Length [aa] | 153 |
| Molecular Weight | 17.5 kDa |
| Species Reactivity | Human, Monkey, Pig, Rat, Mouse |
| Formulation | lyophilized |
| Buffer | 10mM Sodium Citrate, pH 4.0 |
| Protein Sequence | MVPIRQLHYR LRDEQQKSLV LSDPYELKAL HLNGQNINQQ VIFSMSFVQG EPSNDKIPVA LGLKGKNLYL SCVMKDGTPT LQLESVDPKQ YPKKKMEKRF VFNKIEVKSK VEFESAEFPN WYISTSQAEH KPVFLGNNSG QDIIDFTMES VSS |
| Reconstitution | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carri |
| Stability and Storage | The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 6 months at -20°C to -80°C. |
| Synonyms | Il1b; Il-1b; IL-1beta |
| Uniprot ID | P10749 |
| Protein RefSeq | NP_032387 |
| mRNA RefSeq | NM_008361 |