Human holo-Transferrin (Animal free) protein Human 1000 µg
Description / Human holo-Transferrin (Animal free) protein
Transferrin (TF) is found in vertebrate that mediates the transport of iron through blood plasma. When not bound to iron, the protein is known as apo-Transferrin. Each molecule contains two binding sites for iron in the ferric form Fe(III). The iron-bound holo-Transferrin (HTF) can bind to the dimeric Transferrin receptor (TfR) on a cell’s surface and is subsequently internalized by endocytosis. A reduction of pH in the vesicle decreases its iron-affinity and causes HTF to release bound iron ions. Transferrin appears to be essential for most cells growing in tissue culture. ReliaTech's Recombinant Human holo-Transferrin is a 76 kDa glycoprotein consisting of 679 amino acid residues.
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| Size | 1000 µg |
|---|---|
| Source | HEK 293 cells |
| Biological Activity | The biological activity is determined by the stimulation of cell proliferation of MDCK cells in serum-free cell culture medium. |
| Purity Confirmation | ≥ 98% by SDS-PAGE |
| Endotoxin | >= 1200 ppm |
| Length [aa] | 679 |
| Molecular Weight | 76 kDa |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS (pH 7.4) |
| Protein Sequence | VPDKTVRWCA VSEHEATKCQ SFRDHMKSVI PSDGPSVACV KKASYLDCIR AIAANEADAV TLDAGLVYDA YLAPNNLKPV VAEFYGSKED PQTFYYAVAV VKKDSGFQMN QLRGKKSCHT GLGRSAGWNI PIGLLYCDLP EPRKPLEKAV ANFFSGSCAP CADGTDFPQL CQLCPGCGCS TLNQYFGYSG AFKCLKDGAG DVAFVKHSTI FENLANKADR DQYELLCLDN TRKPVDEYKD CHLAQVPSHT VVARSMGGKE DLIWELLNQA QEHFGKDKSK EFQLFSSPHG KDLLFKDSAH GFLKVPPRMD AKMYLGYEYV TAIRNLREGT CPEAPTDECK PVKWCALSHH ERLKCDEWSV NSVGKIECVS AETTEDCIAK IMNGEADAMS LDGGFVYIAG KCGLVPVLAE NYNKSDNCED TPEAGYFAVA VVKKSASDLT WDNLKGKKSC HTAVGRTAGW NIPMGLLYNK INHCRFDEFF SEGCAPGSKK DSSLCKLCMG SGLNLCEPNN KEGYYGYTGA FRCLVEKGDV AFVKHQTVPQ NTGGKNPDPW AKNLNEKDYE LLCLDGTRKP VEEYANCHLA RAPNHAVVTR KDKEACVHKI LRQQQHLFGS NVTDCSGNFC LFRSETKDLL FRDDTVCLAK LHDRNTYEKY LGEEYVKAVG NLRKCSTSSL LEACTFRRP |
| Reconstitution | Centrifuge the vial prior to opening. Do not vortex. The lyophilized HTF should be reconstituted in water to a concentration of 1.0 mg/mL. |
| Stability and Storage | The lyophilized HTF is stable at room temperature for 1 month and at 2 °C to 8 °C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C. |
| Application | cell culture |
| Synonyms | HTF, Serotransferrin, Transferrin, TF, Holo Transferrin, Beta-1 metal-binding-globulin, Siderophilin |
| Uniprot ID | P02787 |
| Protein RefSeq | NP_001054.2 |
| mRNA RefSeq | NM_001063.4 |