Description / E-Selectin
Selectins are a family of calcium-dependent type 1 transmembrane proteins. Endothelial (E)-selectin is a heavily glycosylated transmembrane protein expressed by activated endothelial cells in microvascular linings. E-selectin, along with P-selectin and L-selectin, initiate recruitment of circulating leukocytes from blood to sites of inflammation in the vascular lining through interaction with specific cell surface associated carbohydrate determinants. E-selectin consists of an N-terminal type 1 lectin domain, an EGF-like domain, 6 sushi (CCP/SCR) domains, a transmembrane sequence, and a short cytoplasmic domain. Recombinant human E-selectin is a 58.6 kDa protein containing 535 amino acid residues, corresponding to the extracellular portion of the full length protein. Due to glycosylation, E-selectin migrates at an apparent molecular weight of approximately 65-85 kDa by SDS-PAGE analysis under reducing conditions.
More Information
Size | 10 µg |
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Source | CHO cells |
Biological Activity | Measured by its ability to support adhesion of U937 cells, a human hystiocytic lymphoma cell line. |
Purity Confirmation | >95% by SDS-PAGE & HPLC analysis |
Length [aa] | 535 |
Molecular Weight | 58.6 kDa |
Species Reactivity | Human |
Formulation | lyophilized |
Protein Sequence | WSYNTSTEAM TYDEASAYCQ QRYTHLVAIQ NKEEIEYLNS ILSYSPSYYW IGIRKVNNVW VWVGTQKPLT EEAKNWAPGE PNNRQKDEDC VEIYIKREKD VGMWNDERCS KKKLALCYTA ACTNTSCSGH GECVETINNY TCKCDPGFSG LKCEQIVNCT ALESPEHGSL VCSHPLGNFS YNSSCSISCD RGYLPSSMET MQCMSSGEWS APIPACNVVE CDAVTNPANG FV |
Synonyms | Endothelial leukocyte adhesion molecule 1, ELAM1, CD62E, SELE |
Uniprot ID | P16581 |
Protein RefSeq | NP_000441.2 |
mRNA RefSeq | NM_000450.2 |