Description / E-Selectin
E-Selectin (Endothelial Leukocyte Adhesion Molecule1, ELAM1, CD62E), a member of the Selectin family, is a 107-115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL 1β and TNFα, and demonstrates peak expression at 4 hours, and decay at 24 hours, in response to activation. E-Selectin ligands, expressed on neutrophils, monocytes, and a subset of memory T cells, are sialylated, fucosylated molecules which bind to the lectin domain of E-Selectin. Immunocytochemical techniques have demonstrated the expression of E-Selectin on healthy and diseased tissue. The human and mouse E-Selectin proteins share 81% amino acid similarity. E-Selectin mediates the attachment of flowing leukocytes to the blood vessel wall during inflammation by binding to E-Selectin ligands on leukocytes. These interactions are labile and permit leukocytes to roll along the vascular endothelium in the direction of blood flow. This initial interaction is followed by a stronger interaction involving ICAM-1 and VCAM-1 that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.
More Information
Size | 100 µg |
---|---|
Source | Mouse |
Isotype | IgG1 |
Clone Nr. | (#9E29) |
Species Reactivity | Human |
Formulation | lyophilized |
Buffer | PBS |
Reconstitution | Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml. |
Stability and Storage | Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity. |
Preparation | This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with purified human recombinant E-selectin protein. The IgG2 fraction of the tissue culture supernatant was purified by Protein G affinity chromatogr |
Antigen | recombinant human E-Selectin |
Application | FC, IP, Detection Ab |
Synonyms | SELE; ELAM; ESEL; CD62E; ELAM1; LECAM2 |
Uniprot ID | P16581 |
Protein RefSeq | NP_000441.2 |
mRNA RefSeq | NM_000450 |