E-Selectin Anti-Human 100 µg

In stock

Cat-Nr.
101-M685
Size
100 µg
  €533.00

Description / E-Selectin

E-Selectin (Endothelial Leukocyte Adhesion Molecule1, ELAM1, CD62E), a member of the Selectin family, is a 107-115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL 1β and TNFα, and demonstrates peak expression at 4 hours, and decay at 24 hours, in response to activation. E-Selectin ligands, expressed on neutrophils, monocytes, and a subset of memory T cells, are sialylated, fucosylated molecules which bind to the lectin domain of E-Selectin. Immunocytochemical techniques have demonstrated the expression of E-Selectin on healthy and diseased tissue. The human and mouse E-Selectin proteins share 81% amino acid similarity. E-Selectin mediates the attachment of flowing leukocytes to the blood vessel wall during inflammation by binding to E-Selectin ligands on leukocytes. These interactions are labile and permit leukocytes to roll along the vascular endothelium in the direction of blood flow. This initial interaction is followed by a stronger interaction involving ICAM-1 and VCAM-1 that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.

More Information

Size 100 µg
Source Mouse
Isotype IgG1
Clone Nr. (#9E29)
Species Reactivity Human
Formulation lyophilized
Buffer PBS
Reconstitution Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.
Stability and Storage Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity.
Preparation This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with purified human recombinant E-selectin protein. The IgG2 fraction of the tissue culture supernatant was purified by Protein G affinity chromatogr
Antigen recombinant human E-Selectin
Application FC, IP, Detection Ab
Synonyms SELE; ELAM; ESEL; CD62E; ELAM1; LECAM2
Uniprot ID P16581
Protein RefSeq NP_000441.2
mRNA RefSeq NM_000450

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