Description / anti-mouse VEGFR-3/FLT-4 monoclonal antibody
Receptor tyrosine kinase VEGFR-3, also known as Flt-4, together with VEGFR-1 (Flt-1) and VEGFR-2 (KDR/Flk-1), are the receptors for vascular endothelial growth factors (VEGF). The VEGFR family belongs to the class II subfamily of receptor tyrosine kinases (RTKs), containing a large extracellular region which is composed of seven Ig-like domains (D1–D7), a single transmembrane (TM) helix and cytoplasmic region with a tyrosine kinase activity. In VEGFR-3, the fifth Ig homology domain is proteolytically cleaved which results in polypeptides remaining linked by two disulfide bonds. VEGFR-3 is widely expressed on all endothelial cells in early embryogenesis, while, in adult tissues, VEGFR-3 expression disappears from the vascular endothelial cells and is observed only on the lymphatic endothelium. VEGF-C and VEGF-D activation of VEGFR-3 plays an important role in the formation of the lymphatic vessel system.
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| Size | 100 µg |
|---|---|
| Source | Rat |
| Isotype | IgG2 |
| Biological Activity | Western blot: Use at 0.25 - 0.50 µg/ml; IHC (Paraffin sections): Use at 0.4 - 2.0 µg/ml. |
| Clone Nr. | (#5F63) |
| Species Reactivity | No cross reactivity to mouse VEGFR-1 and VEGFR-2. Cross reactivity to other species has not been tested! |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml. |
| Stability and Storage | Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity. |
| Preparation | This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat) immunized with mouse VEGFR-3 extracellular domain. IgG fraction of the tissue culture supernatant was purified by Protein G affinity chromatography. |
| Antigen | Mouse VEGFR-3 extracellular domain |
| Application | WB, IHC (P) |
| Synonyms | vascular endothelial growth factor receptor-3; Flt4; Chy; Flt-4; VEGFR-3 |
| Uniprot ID | P35917 |
| Protein RefSeq | NP_032055.1 |
| mRNA RefSeq | NM_008029 |