Description / anti-mouse VEGFR-3/FLT-4 monoclonal antibody
VEGFR-3, also known as FLT4, is a member of the Tyr protein kinase family. The extracellular portion of VEGFR-3 contains 7 immunoglobulin (Ig)-like domains and the cytoplasmic portion contains a protein kinase domain. FLT4 regulates angiogenesis and lymphangiogenesis, its ligands are VEGF-C and D and its binding is mediated by the 2nd and 3rd Ig-like domains of FLT4. During fetal development VEGFR-3 is expressed on endothelial cells, however, in the adult mice, the vascular endothelial cells lose VEGFR-3 expression, but the lymphatic endothelium expresses it constitutively. In addition, VEGFR-3 expression can be induced in tumors with active angiogenesis.
More Information
| Size | 100 µg |
|---|---|
| Source | Rat |
| Biological Activity | Western Blot: use at 1-5 µg/ml. |
| Clone Nr. | (#7A3) |
| Species Reactivity | Mouse |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% |
| Preparation | The hybridoma was generated after fusion with cells from rat immunized with purified, insect cell-derived recombinant mouse soluble FLT-4 (120 kDa) as the immunizing antigen. Rat IgG1 antibody from hybridomas was purified from cell culture supernatant by |
| Antigen | recombinant mouse soluble FLT-4 |
| Application | WB |
| Synonyms | vascular endothelial growth factor receptor-3; Flt4; Chy; Flt-4; VEGFR3; VEGFR-3 |
| Uniprot ID | P35917 |
| Protein RefSeq | NP_032055.1 |
| mRNA RefSeq | NM_008029 |