Description / anti-human VEGF-C monoclonal antibody
VEGF-C, a member of the VEGF/PDGF family of structurally related proteins, is a potent angiogenic cytokine. It promotes endothelial cell growth, promotes lymphangiogesis, and can also affect vascular permeability. VEGF-C is expressed in various tissues, but is not produced in peripheral blood lymphocytes. It forms cell surfaced-associated non-covalent disulfide linked homodimers, and can bind and activate both VEGFR-2 (flk1) and VEGFR-3 (flt4) receptors. During embryogenesis, VEGF-C may play a role in the formation of the venous and lymphatic vascular systems. Both VEGF-C and VEGF-D are over-expressed in certain cancers, and the resulting elevated levels of VEGF-C or VEGF-D tend to correlate with increased lymphatic metastasis. Recombinant, fully processed human VEGF-C is a 13.5 kDa non-disulfide linked homodimeric protein consisting of two 116 amino acid polypeptide chains. Due to glycosylation the protein migrates as a 20.0-22.0 kDa band under non-reducing condition.
More Information
| Size | 50 µg |
|---|---|
| Source | Mouse |
| Isotype | IgG2 |
| Biological Activity | WB: 1:1000; IHC (Formalin/Paraffin): 1:25-50 |
| Clone Nr. | (#2E65) |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml. |
| Stability and Storage | Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity. |
| Preparation | This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with recombinant human VEGF-C recombinant protein). The IgG2 fraction of culture supernatant was purified by Protein G affinity chromatography. |
| Antigen | Recombinant human VEGF-C |
| Application | WB, IHC (P) |
| Synonyms | vascular endothelial growth factor C; VEGFC; VRP; Flt4-L |
| Uniprot ID | P49767 |
| Protein RefSeq | NP_005420.1 |
| mRNA RefSeq | NM_005429.2 |