Description / anti-human VE-Cadherin monoclonal antibody
The antibody reacts with human CD144, also known as VE-cadherin and cadherin-5. The cadherin family of receptors, which are calcium-dependent adhesion molecules, is known to be involved in homophilic cell interactions. VE-cadherin, which is 140 kDa, is localized at the intercellular boundaries of endothelial cells in blood and lymphatic vessels in several tissues. It is thought to play a role in vascular permeability and remodeling.
More Information
| Size | 100 µg |
|---|---|
| Source | Mouse |
| Isotype | IgG1 |
| Biological Activity | WB: 1:1000-2000; ELISA: 1:1000-3000; IHC: 1:100-200 |
| Clone Nr. | (#8A03) |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml. |
| Stability and Storage | Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity. |
| Preparation | This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse) immunized with human VE-Cadherin recombinant protein. The IgG1 fraction of culture supernatant was purified by Protein G affinity chromatography. |
| Antigen | extracellular domain of human VE-Cadherin |
| Application | ELISA, WB, IHC |
| Synonyms | CDH5; 7B4; CD144 |
| Uniprot ID | P33151 |
| Protein RefSeq | NP_001786.2 |
| mRNA RefSeq | NM_001795.3 |