Description / anti-mouse TNF-alpha polyclonal antibody
TNF-α is a pleiotropic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-α also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes. Recombinant murine TNF-α is a soluble 156 amino acid protein (17.3 kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein.
More Information
| Size | 100 µg |
|---|---|
| Source | Goat |
| Clone Nr. | Goat IgG |
| Species Reactivity | Mouse |
| Formulation | lyophilized from PBS |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. |
| Preparation | Produced from sera of goats pre-immunized with highly pure (>98%) recombinant mTNF-alpha. Anti-mTNF-alpha specific antibody was purified by affinity chromatography employing immobilized mTNF-alpha matrix. |
| Antigen | recombinant mouse TNF-alpha |
| Application | ELISA, WB, N |
| Synonyms | Tnf; DIF; Tnfa; TNFSF2; Tnfsf1a; TNFalpha; TNF-alpha |
| Uniprot ID | P06804 |
| Protein RefSeq | NP_038721.1 |
| mRNA RefSeq | NM_013693.2 |