Description / anti-human TNF-alpha polyclonal antibody
Tumor necrosis factor alpha (TNF-α), also known as cachectin and TNFSF1A, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-α is produced by a wide variety of immune and epithelial cell types. Human TNF-α consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD). Within the ECD, human TNF-α shares 97% aa sequence identity with rhesus and 71% - 92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-α. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer. Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness.
More Information
| Size | 100 µg |
|---|---|
| Source | Goat |
| Clone Nr. | Goat IgG |
| Species Reactivity | Human |
| Formulation | lyophilized from PBS |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. |
| Preparation | Produced from sera of goats pre-immunized with highly pure (>98%) recombinant human TNF alpha (human Tumor Necrosis Factor-alpha). Anti-human TNF alpha specific antibody was purified by affinity chromatography employing immobilized human TNF alpha matrix. |
| Antigen | recombinant human TNF-alpha |
| Application | ELISA, WB, N |
| Synonyms | TNF; DIF; TNFA; TNFSF2; TNF-alpha |
| Uniprot ID | P01375 |
| Protein RefSeq | NP_000585.2 |
| mRNA RefSeq | NM_000594 |