Description / anti-human TIMP-1 polyclonal antibody
TIMPs-1 through -4 regulate the activity of zinc metalloproteases known as MMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin- like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation of MMP-2 by MMP-14.
More Information
| Size | 100 µg |
|---|---|
| Source | Rabbit |
| Clone Nr. | Rabbit IgG |
| Species Reactivity | Human |
| Formulation | lyophilized from PBS |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. |
| Preparation | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant human TIMP-1 (human Tissue Inhibitor of Metalloproteinases-1). Anti-Human TIMP-1 specific antibody was purified by affinity chromatography employing immobilized human TIMP-1 m |
| Antigen | recombinant human TIMP-1 |
| Application | ELISA, WB |
| Synonyms | TIMP1; EPA; EPO; HCI; CLGI; TIMP |
| Uniprot ID | P01033 |
| Protein RefSeq | NP_003245 |
| mRNA RefSeq | NM_003254 |