Description / anti-human TIMP-1 monoclonal antibody
TIMPs-1 through -4 regulate the activity of zinc metalloproteases known as MMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin- like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation of MMP-2 by MMP-14.
More Information
| Size | 50 µg |
|---|---|
| Source | Mouse |
| Isotype | IgG2 |
| Clone Nr. | (#4A16) |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml. |
| Stability and Storage | Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity. |
| Preparation | This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with purified recombinant human tissue inhibitor of metalloproteinases-1 (TIMP-1). The IgG fraction of the tissue culture supernatant was purified by |
| Antigen | human recombinant TIMP-1 protein |
| Application | WB, IHC (P) |
| Synonyms | TIMP1; EPA; EPO; HCI; CLGI; TIMP |
| Uniprot ID | P01033 |
| Protein RefSeq | NP_003245 |
| mRNA RefSeq | NM_003254 |