Description / anti-mouse T-cell Immunoglobulin Mucin-3 (TIM-3) monoclonal antibody
T cell immunoglobulin mucin-3 (TIM-3) is a member of the T-cell Immunoglobulin- and Mucin-domain-containing family of type I membrane glycoproteins that regulate autoimmune and allergic disease. TIM-3 is selectively expressed on Th1 cells and interacts with galectin-9. It negatively regulates Th1 responses and affects macrophage activation. The 280 aa mature human TIM-3 contains a V-type Ig-like domain that shows multiple polymorphisms, followed by a mucin-like domain in the 171 aa extracellular region, which shares 60% amino acid identity with mouse TIM-3 ECD. One splice variant of TIM-3 is truncated within the mucin domain and presumably is secreted.
More Information
| Size | 100 µg |
|---|---|
| Source | Rat |
| Isotype | IgG2 |
| Clone Nr. | (#6F46) |
| Species Reactivity | Mouse |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml. |
| Stability and Storage | Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity. |
| Preparation | This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with purified mouse recombinant TIM-3. The IgG2 fraction of the culture supernatant was purified by Protein A/G affinity chromatography. |
| Antigen | mouse recombinant protein of TIM-3 |
| Application | WB |
| Synonyms | Havcr2; Tim3; TIM-3; Timd3 |
| Uniprot ID | Q8VIM0 |
| Protein RefSeq | NP_599011.2 |
| mRNA RefSeq | NM_134250.2 |