Description / anti-mouse T-cell Immunoglobulin Mucin-1 (TIM-1) monoclonal antibody
MUC-1 (Mucin-1; also PEM, PEMT, Episialin, tumor-associated mucin, and carcinoma associated mucin) is a 240-450 kDa type I transmembrane glycoprotein. Human MUC-1 is 1255 amino acids (aa) in length and contains a 23 aa signal sequence, a 1135 aa extracellular domain (ECD), a 23 aa transmembrane segment, and a 74 aa cytoplasmic tail. The ECD consists of degenerate tandem repeats and a tandem repeat region, which makes up the major portion of the protein. Potential O-glycosylation sites (serines and threonines) make up more than one-fourth of the amino acids. Splicing variants produce ten isoforms for human MUC-1. Human MUC-1 is 28% aa identical to mouse MUC-1.
More Information
| Size | 50 µg |
|---|---|
| Source | Rat |
| Isotype | IgG2 |
| Clone Nr. | (#3F22) |
| Species Reactivity | Mouse |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml. |
| Stability and Storage | Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity. |
| Preparation | This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with purified mouse recombinant TIM-1. The IgG2 fraction of the culture supernatant was purified by Protein A/G affinity chromatography. |
| Antigen | mouse recombinant protein of TIM-1 |
| Application | WB |
| Synonyms | Havcr1; Tim1; KIM-1; TIM-1; Timd1; AI503787 |
| Uniprot ID | Q5QNS5 |
| Protein RefSeq | NP_599009.2 |
| mRNA RefSeq | NM_134248.2 |