Description / anti-mouse Stem Cell Antigen 1/Sca-1 monoclonal antibody
Sca-1 is an 18 kDa phosphatidylinositol-anchored protein that is a member of the lymphocyte antigen 6 (Ly-6) family. Sca-1 is encoded by the strain-specific Ly-6 E/A allelic gene. Its expression on multipotent hematopoietic stem cells (HSC) has been used as a marker of HSCs in mice of both Ly-6 haplotypes. Sca-1-positive HSCs can be found in the adult bone marrow, fetal liver, and mobilized peripheral blood and spleen in the adult animal. However, Sca-1 has also been discovered in several non-hematopoietic tissues and can be used to enrich other progenitor cell populations as well. It is suggested that Sca-1 could be involved in regulating both B and T cell activation.
More Information
| Size | 100 µg |
|---|---|
| Source | Rat |
| Isotype | IgG2 |
| Clone Nr. | (#2M39) |
| Species Reactivity | Mouse |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml. |
| Stability and Storage | Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity. |
| Preparation | This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat) immunized with mouse recombinant protein of Sca-1. |
| Antigen | Mouse recombinant protein of Sca-1 |
| Application | IHC (F) |
| Synonyms | Ly6a; TAP; Sca1; Sca-1; Ly-6A.2; Ly-6A/E; Ly-6E.1 |
| Uniprot ID | P05533.1 |
| Protein RefSeq | NP_034868.1 |
| mRNA RefSeq | NM_010738.3 |