Description / anti-mouse SPARC monoclonal antibody
SPARC, also known as Osteonectin and Basement Membrane protein BM-40, is a phosphorylated glycoprotein with calcium-binding domains. It is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. SPARC may be involved in cell proliferation, repair of tissue damage, and morphogenic processes such as re-modeling of the extracellular matrix.
More Information
| Size | 100 µg |
|---|---|
| Source | Rat |
| Isotype | IgG2 |
| Clone Nr. | (#5F41) |
| Species Reactivity | Mouse |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml. |
| Stability and Storage | Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity. |
| Preparation | This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with purified mouse recombinant SPARK. The IgG2 fraction of the culture supernatant was purified by Protein A/G affinity chromatography. |
| Antigen | recombinant mouse SPARC |
| Application | ELISA, IHC (F) |
| Synonyms | Sparc; BM-40; AA517111 |
| Uniprot ID | P07214 |
| Protein RefSeq | NP_033268.1 |
| mRNA RefSeq | NM_009242.4 |