Description / mouse R-Spondin-1 protein
R-Spondin-1 (Rspo-1) belongs to the (Rspo) family of Wnt modulators. Currently, the family consists of four structurally related secreted ligands (Rspo 1-4), all containing the furin-like and thrombospondin structural domains. Rspo-1 is expressed in certain areas of the developing central nervous system, as well as in the adrenal glands, ovary, testis, thyroid, and trachea. Rspo can interact with the Frizzled/LRP6 receptor complex in a manner that stimulates the Wnt/beta-catenin signaling pathway. Recombinant Murine R-Spondin-1 is a 27.1 kDa protein consisting of 245 amino acid residues. Due to glycosylation, R-Spondin-1 migrates at an apparent molecular weight of approximately 40.0 kDa by SDS-PAGE analysis under reducing conditions.
More Information
| Size | 5 µg |
|---|---|
| Source | CHO cells |
| Biological Activity | R-Spondin-1 enhances BMP-2-mediated differentiation of MC3T3-E1 cells. |
| Purity Confirmation | > 95% by SDS-PAGE & HPLC analyses |
| Length [aa] | 245 |
| Molecular Weight | 40.0 kDa |
| Species Reactivity | Mouse |
| Formulation | lyophilized |
| Buffer | 10mM Sodium Phosphate, pH 7.5 and 150mM NaCl |
| Protein Sequence | SRGIKGKRQR RISAEGSQAC AKGCELCSEV NGCLKCSPKL FILLERNDIR QVGVCLPSCP PGYFDARNPD MNKCIKCKIE HCEACFSHNF CTKCQEGLYL HKGRCYPACP EGSTAANSTM ECGSPAQCEM SEWSPWGPCS KKRKLCGFRK GSEERTRRVL HAPGGDHTTC SDTKETRKCT VRRTPCPEGQ KRRKGGQGRR ENANRHPARK NSKEPGSNSR RHKGQQQPQP GT |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Do not vortex. |
| Synonyms | Roof plate-specific Spondin-1, RSPO1 |
| Uniprot ID | Q9Z132 |
| Protein RefSeq | NP_619624.2 |
| mRNA RefSeq | NM_138683.2 |