Description / human PAF-AH protein
Platelet Activating Factor (PAF) is a biologically active phospholipid, which exerts primarily proinflammatory activities by specifically signaling through G-protein-coupled receptors on platelets, neutrophils, and monocytes. Platelet Activating Factor Acetylhydrolase (PAF-AH) is a secreted protein that mediates PAF activity by specifically catalyzing hydrolysis of the “sn2” ester bond, resulting in the conversion of PAF to the biologically inactive lyso-PAF. PAF-AH can also interact with LDL particles to induce the hydrolysis of LDL associated, oxidized phospholipids, generating lysophosphatidylcholine (lyso-PC) and other lysophospholipids. Recombinant PAF-AH is a 420 amino acid glycoprotein which migrates with an apparent molecular mass of 47-55 kDa by SDS-PAGE analysis.
More Information
| Size | 20 µg |
|---|---|
| Source | HEK 293 cells |
| Biological Activity | Measured by its ability to cleave a PAF analog in a chromogenic substrate linked assay. At a PAF-AH concentration of 10.0 μg/ml, 50% cleavage was achieved at an incubation time of approximately 2 minutes. |
| Purity Confirmation | > 95% by SDS-PAGE & HPLC analyses |
| Length [aa] | 420 |
| Molecular Weight | 47-55 kDa |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Protein Sequence | FDWQYINPVA HMKSSAWVNK IQVLMAAASF GQTKIPRGNG PYSVGCTDLM FDHTNKGTFL RLYYPSQDND RLDTLWIPNK EYFWGLSKFL GTHWLMGNIL RLLFGSMTTP ANWNSPLRPG EKYPLVVFSH GLGAFRTLYS AIGIDLASHG FIVAAVEHRD RSASATYYFK DQSAAEIGDK SWLYLRTLKQ EEETHIRNEQ VRQRAKECSQ ALSLILDIDH GKPVKNALDL KF |
| Synonyms | PAF-acetylhydrolase, PAF 2-acylhydrolase, LDL-PLA82), 2-acetyl-1-alkylglycerophosphacholine esterase |
| Uniprot ID | P68402 |
| Protein RefSeq | NP_002563.1 |
| mRNA RefSeq | NM_002572.3 |