Description / anti-human MMP-8 monoclonal antibody
Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP8 (neutrophil collagenase) is expressed in neutrophils, where it is stored in specific granules. MMP8 release from the neutrophils is stimulated by various factors such as interleukins 1 and 8, TNFα and GM-CSF. MMP8 is capable of cleaving types I, II and III triplehelical collagen, gelatin peptides, fibronectin, proteoglycans, aggrecan, serpins, βcasein and peptides such as angiotensin and substance P. In addition to its function in phagocytosis, MMP8 has a high capacity for infiltrating connective tissue, and is implicated in the breakdown of the extracellular matrix in diseases such as rheumatoid arthritis. Structurally, MMP8 consists of several domains: a prodomain that is cleaved upon activation, a catalytic domain containing the zinc-binding site, a short hinge region and a hemopexinlike domain. MMP8 is heavily glycosylated.
More Information
| Size | 100 µg |
|---|---|
| Source | Mouse |
| Isotype | IgG2 |
| Biological Activity | WB: Use at 1:400-1000; IHC: 1:50-200 |
| Clone Nr. | (#7A11) |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml. |
| Stability and Storage | Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity. |
| Preparation | This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with purified human recombinant human MMP-12). The IgG2 fraction of the tissue culture supernatant was purified by Protein G affinity chromatography. |
| Antigen | recombinant human MMP-8 |
| Application | WB, IHC (P) |
| Synonyms | MMP8; HNC; CLG1; MMP-8; PMNL-CL |
| Uniprot ID | P22894 |
| Protein RefSeq | NP_002415.1 |
| mRNA RefSeq | NM_002424 |