Description / anti-human MMP-15 monoclonal antibody
Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. They play critical roles in tissue remodeling, angiogenesis, tumor invasion, and rheumatoid arthritis. MMP15, also known as MT2MMP, is a membranetype MMP that is expressed in many tumor tissues including urothelial carcinoma, oral cancer, ovarian carcinoma, melanoma, and astrocytoma. Structurally, MMP15 consists of the following domains:a pro domain containing a furin cleavage site, a catalytic domain containing the zinc-binding site, a hinge region, a hemopexinlike domain, a transmembrane domain, and a cytoplasmic tail. Recombinant Human (rh) MMP15, consists of the pro domain, catalytic domain, hinge region and hemopexin-like domain. The pro domain contains the mutations R128P and R129G, which prevent activation by furin cleavage. Activation of rhMMP15 is possible by treatment with rhTrypsin.
More Information
| Size | 100 µg |
|---|---|
| Source | Mouse |
| Isotype | IgG1 |
| Clone Nr. | (#5D79) |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml. |
| Stability and Storage | Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity. |
| Preparation | This antibody was produced from a hybridoma (mouse myeloma fused with pleen cells from a mouse immunized with purified human recombinant human matrix metalloproteinase-15). The IgG1 fraction of the tissue culture supernatant was purified by Protein G affi |
| Antigen | recombinant human MMP-15 |
| Application | WB, IHC (P), IP, Detection Ab |
| Synonyms | MMP15; MTMMP2; SMCP-2; MT2-MMP |
| Uniprot ID | P51511 |
| Protein RefSeq | NP_002419.1 |
| mRNA RefSeq | NM_002428.2 |