Description / anti-human MMP-13 monoclonal antibody
Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP13 (Collagenase-3) has been demonstrated to degrade a range of extracellular matrix proteins, including collagen types I, II, III, IV, IX, X and XIV, gelatin, aggrecan, perlecan and fibronectin. MMP-13 is distinguished from the other human collagenases by its effecient degradation of type II collagen. MMP13 is expressed by fibroblasts, chrondrocytes and squamous epithelial cells. Structurally, MMP-13 may be divided into several distinct domains; a prodomain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexinlike) domain.
More Information
| Size | 100 µg |
|---|---|
| Source | Mouse |
| Isotype | IgG1 |
| Biological Activity | WB: Use at 1:400-1000; IHC: 1:50-200 |
| Clone Nr. | (#12E10) |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml. |
| Stability and Storage | Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity. |
| Preparation | This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with purified human recombinant human MMP-13). The IgG1 fraction of the tissue culture supernatant was purified by Protein G affinity chromatography. |
| Antigen | recombinant human MMP-13 |
| Application | WB, IHC (P) |
| Synonyms | MMP13; CLG3; MANDP1 |
| Uniprot ID | P45452 |
| Protein RefSeq | NP_002418.1 |
| mRNA RefSeq | NM_002427.3 |