Description / anti-human MMP-12 monoclonal antibody
MMP12, also known as macrophage elastase, is a zinc and calcium dependent endopeptidase expressed constitutively in macrophages and can be induced in monocytes. MMP12 can degrade a broad spectrum of substrates, including type IV collagen, fibronectin, laminin, vitronectin, proteoglycans, chondroitin sulfate, myelin basic protein, α-1antitrypsin, and plasminogen. It can also activate MMP2 and MMP-3. The MMP12 precursor consists of a pro domain, a catalytic domain containing the zinc-binding site, and a C-terminal hemopexin like domain.
More Information
| Size | 100 µg |
|---|---|
| Source | Mouse |
| Isotype | IgG2 |
| Biological Activity | WB: Use at 1:500-2000; IHC: 1:50-300 |
| Clone Nr. | (#7G20) |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml. |
| Stability and Storage | Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity. |
| Preparation | This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with purified human recombinant human MMP-12). The IgG2 fraction of the tissue culture supernatant was purified by Protein G affinity chromatography. |
| Antigen | recombinant human MMP-12 |
| Application | WB, IHC (P) |
| Synonyms | MMP12; ME; HME; MME; MMP-12 |
| Uniprot ID | P39900 |
| Protein RefSeq | NP_002417.2 |
| mRNA RefSeq | NM_002426.4 |