Description / human Midkine protein
Midkine (MK) and its functionally-related protein Pleiotrophin are heparin-binding neurotrophic factors that signal through the same receptor, known as anaplastic lymphoma kinase (ALK). MK plays an important regulatory role in epithelial-mesenchymal interactions during fetal development and in postnatal lung development. MK chemoattracts embryonic neurons, neutrophils and macrophages, and by signaling through the ALK receptor it exerts angiogenic, growth and survival activities during tumorgenesis. Recombinant human Midkine is a 13.4 kDa protein containing 123 amino acid residues including five intra-molecular disulfide bonds.
More Information
| Size | 5 µg |
|---|---|
| Source | E. coli |
| Biological Activity | Determined by its ability to chemoattract human neutrophils using a concentration range of 0.1-10.0 ng/ml. |
| Purity Confirmation | > 98% by SDS-PAGE & HPLC analyses |
| Length [aa] | 123 |
| Molecular Weight | 13.4 kDa |
| Species Reactivity | Mouse, Pig, Shark, Human, Hagfish, Squid |
| Formulation | lyophilized |
| Buffer | 0.4x PBS, pH 7.4 |
| Protein Sequence | VAKKKDKVKK GGPGSECAEW AWGPCTPSSK DCGVGFREGT CGAQTQRIRC RVPCNWKKEF GADCKYKFEN WGACDGGTGT KVRQGTLKKA RYNAQCQETI RVTKPCTPKT KAKAKAKKGK GKD |
| Reconstitution | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carri |
| Stability and Storage | The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C. |
| Synonyms | MDK; MK; NEGF2 |
| Uniprot ID | P21741 |
| Protein RefSeq | NP_001012333 |
| mRNA RefSeq | NM_001012333 |