Description / anti-mouse Lyve-1 monoclonal antibody
LYVE-1 has been identified as a major receptor for HA (extracellular matrix glycosaminoglycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41% similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE-1 molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE-1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves.
More Information
| Size | 50 µg |
|---|---|
| Source | Rat |
| Isotype | IgG2 |
| Clone Nr. | (#4D17) |
| Species Reactivity | Mouse |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml. |
| Stability and Storage | Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity. |
| Preparation | Produced from a hybridoma fused by a mouse myeloma with B cells obtained from a rat immunized with mouse LYVE-1 recombinant protein. IgG2 fraction of the culture supernatant was purified by Protein G affinity chromatography. |
| Antigen | Mouse recombinant LYVE-1 |
| Application | WB, IHC (F) |
| Synonyms | Lyve1; Xlkd1; Lyve-1; Crsbp-1; 1200012G08Rik |
| Uniprot ID | Q8BHC0 |
| Protein RefSeq | NP_444477 |
| mRNA RefSeq | NM_053247 |