Description / anti-mouse Lefty-1 monoclonal antibody
Lefty proteins are novel TGF-beta ligands that function as antagonists of Nodal signaling. The expression of Lefty proteins on the left side of the developing mouse embryo earned this protein family its name. Two genes exist in mouse (Lefty-1 and Lefty-2) and two in humans (Lefty-A and Lefty-B). By amino acid sequence, human Lefty-A and Lefty-B are more similar to each other (96%) than to either Lefty-1 or Lefty-2 in the mouse (81-82%). The Leftys contains some of the features of the cysteine-knot structure conserved among TGF-beta-related proteins, but also some structural differences. Specifically, they lack the alpha-helical segment important for ligand dimerization as well as the cysteine residue involved in stabilization of dimers.
More Information
| Size | 50 µg |
|---|---|
| Source | Rat |
| Isotype | IgG2 |
| Clone Nr. | (#12A13) |
| Species Reactivity | Mouse |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml. |
| Stability and Storage | Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity. |
| Preparation | This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with purified mouse recombinant protein of Lefty-1. The IgG2 fraction of the culture supernatant was purified by Protein A/G affinity chromatography. |
| Antigen | Recombinant mouse Lefty-1 |
| Application | WB |
| Synonyms | Protein lefty-1, Lefty protein, Stimulated by retinoic acid gene 3 protein, TGF-beta-4, Lefty1, Ebaf, Lefty, Stra3, Tgfb4 |
| Uniprot ID | Q64280 |
| Protein RefSeq | NP_034224.1 |
| mRNA RefSeq | NM_010094.3 |