Description / anti-human Indian Hedgehog monoclonal antibody
Indian Hedgehog (Ihh), Sonic Hedgehog (Shh), and Desert Hedgehog (Dhh) are important signaling molecules during embryonic development and are highly conserved within and across species. Mouse Ihh cDNA encodes a 411 amino acid (aa) polypeptide with a predicted 27 aa signal peptide. Post-translational processing yields a 19 kDa lipid-modified N-terminal domain that is the signaling molecule. At the cell surface, Ihh activity is mediated by binding the 12-pass transmembrane receptor, Patched (Ptc), and signaling through the 7-pass transmembrane G-protein coupled receptor, Smoothened (Smo).
More Information
| Size | 100 µg |
|---|---|
| Source | Rat |
| Isotype | IgG2 |
| Biological Activity | WB: 1:500-1000 |
| Clone Nr. | (#9L16) |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml. |
| Stability and Storage | Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity. |
| Preparation | This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat) immunized with human recombinant Indian Hedgehog N-terminus. |
| Antigen | Recombinant Human Indian Hedgehog N-Terminus |
| Application | WB |
| Uniprot ID | Q14623 |
| Protein RefSeq | NP_002172.2 |
| mRNA RefSeq | NM_002181.3 |