Description / human IL-4 receptor alpha (CD124), soluble protein
IL-4 can signal through type I and type II receptor complexes, which share a common gamma chain. The type I receptor contains, in addition to the gamma chain, an IL-4R alpha subunit, whereas the type II receptor contains the IL-13R alpha. The secreted extracellular domain of IL-4R alpha, called sIL-4R alpha, binds IL-4 and antagonizes its activity. It plays an important role in regulating the differentiation of naïve CD4+ T cells and class switching to IgG1 and IgE. Recombinant Human sIL-4 Receptor alpha is a 23.9 kDa glycoprotein corresponding to 209 amino acid residues of the extracellular domain of IL-4R alpha. As a result of glycosylation, Recombinant Human sIL-4 Receptor alpha migrates with an apparent molecular mass of approximately 50-65 kDa by SDS-PAGE gel, under reducing conditions.
More Information
| Size | 20 µg |
|---|---|
| Source | CHO cells |
| Biological Activity | The ED50 was determined by its ability to inhibit the IL-4 dependent proliferation of human TF-1 cells is ≤ 5.0 ng/ml (in the presence of 0.5 ng/ml of IL-4), corresponding to a specific activity of ≥ 2 x 105 units/mg. |
| Purity Confirmation | > 95% by SDS-PAGE & HPLC analyses |
| Length [aa] | 209 |
| Molecular Weight | 23.9 kDa |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Protein Sequence | GNMKVLQEPT CVSDYMSIST CEWKMNGPTN CSTELRLLYQ LVFLLSEAHT CIPENNGGAG CVCHLLMDDV VSADNYTLDL WAGQQLLWKG SFKPSEHVKP RAPGNLTVHT NVSDTLLLTW SNPYPPDNYL YNHLTYAVNI WSENDPADFR IYNVTYLEPS LRIAASTLKS GISYRARVRA WAQCYNTTWS EWSPSTKWHN SYREPFEQH |
| Synonyms | soluble Interleukin-4Rα, soluble IL-4 Receptor alpha, CD124 |
| Uniprot ID | P24394 |
| Protein RefSeq | NP_000409.1 |
| mRNA RefSeq | NM_000418 |