Description / anti-human IL-33 polyclonal antibody
IL-33 is a proinflammatory protein that may also regulate gene transcription. IL-33 was identified based on sequence and structural homology with IL-1 family cytokines and is constitutively expressed in smooth muscle and airway epithelia. It is upregulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1alpha or IL-1 beta stimulation. Similar to IL-1, IL-33 can be cleaved in vitro by caspase-1, generating an N-terminal fragment that is slightly shorter than the C-terminal fragment. The N-terminal portion of full length IL-33 contains a predicted bipartite nuclear localization sequence and a homeodomain-like helix-turn-helix DNA binding domain.
More Information
| Size | 100 µg |
|---|---|
| Source | Rabbit |
| Clone Nr. | Rabbit IgG |
| Species Reactivity | Human |
| Formulation | lyophilized from PBS |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. |
| Preparation | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant human IL-33. Anti-Human IL-33 specific antibody was purified by affinity chromatography employing immobilized human IL-33 matrix. |
| Antigen | Recombinant human IL-33 |
| Application | ELISA, WB |
| Synonyms | IL33; DVS27; IL1F11; NF-HEV; NFEHEV; C9orf26; RP11-575C20.2 |
| Uniprot ID | O95760 |
| Protein RefSeq | NP_254274 |
| mRNA RefSeq | NM_033439 |