Description / mouse IL-28A protein
IL-28A is distantly related to type I interferons and the IL-10 family. Expression of IL-28A is induced by viral infection which interacts with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha. IL-28A exhibits common features with type I IFNs such as antiviral activity, antiproliferative activity and in vivo antitumour activity. IL-28A acts similarly to IFNs, but is less effective generally and has activity in a more limited range of cell lines. IFN-ambda 1, IFN-lambda 2 and IFN-lambda 3 are closely positioned genes on human chromosome 19. IL-28A induces ELR(-) CXC chemokine mRNA in human peripheral blood mononuclear cells, in an IFN-gamma-independent manner. IL-28A is able to generate tolerogenic DCs, an activity that could thwart IFN-beta functions. IL-28A produced in response to viral infection, activates both monocytes and macrophages producing a restricted panel of cytokines and therefore is an important factor in activating innate immune responses at the site of viral infection.
More Information
| Size | 5 µg |
|---|---|
| Source | E. coli |
| Biological Activity | The ED50 as determined by an anti-viral assay using human HepG2 cells infected with encephalomyocarditis(EMC) virus is less than 3 ng/ml, corresponding to a specific activity of > 3.3 × 105 IU/mg. |
| Purity Confirmation | > 95% by SDS-PAGE & HPLC analyses |
| Length [aa] | 174 |
| Molecular Weight | 19.7kDa |
| Species Reactivity | Mouse |
| Formulation | lyophilized |
| Buffer | PBS/5% Trehalose |
| Protein Sequence | DPVPRATRLP VEAKDCHIAQ FKSLSPKELQ AFKKAKDAIE KRLLEKDMRC SSHLISRAWD LKQLQVQERP KALQAEVALT LKVWENMTDS ALATILGQPL HTLSHIHSQL QTCTQLQATA EPKPPSRRLS RWLHRLQEAQ SKETPGCLED SVTSNLFRLL TRDLKCVASG DQCV |
| Reconstitution | It is recommended to reconstitute the lyophilized IL-28A in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability and Storage | Lyophilized mouse IL-28A although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. |
| Synonyms | Interferon lambda-2, IFN-lambda-2, Interleukin-28A, IL-28A, Ifnl2, Il28a, EG330496 |
| Uniprot ID | Q4VK74 |
| Protein RefSeq | NP_001019844.2 |
| mRNA RefSeq | NM_001024673.2 |