Description / mouse IL-12 p70 protein
IL-12 is a potent regulator of cell-mediated immune responses and it induces IFN-γ production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue-type mast cells. Among its biological activities, IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells, and induces the development of IFN-γ-producing Th1 cells. Recombinant Murine IL-12 is a 75.0 kDa heterodimeric glycoprotein consisting of disulfide-linked 35 kDa (p35) and 40 kDa (p40) subunits (506 total amino acid residues).
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| Size | 2 µg |
|---|---|
| Source | CHO cells |
| Biological Activity | Determined by its ability to induce cell proliferation in T-cell enriched PBMCs. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg. |
| Purity Confirmation | ≥ 98% by SDS-PAGE gel and HPLC analyses |
| Length [aa] | 506 |
| Molecular Weight | 75 kDa |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Protein Sequence | p35 Subunit: RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSA p40 Subunit: MWELEKDVYV VEVDW |
| Synonyms | NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF |
| Uniprot ID | p35: P43431 p40: P43432 |
| Protein RefSeq | NP_032377.1, NP_001290173.1 |
| mRNA RefSeq | NM_008351.4, NM_001303244.1 |