Description / mouse IL-12 protein
Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.
More Information
| Size | 10 µg |
|---|---|
| Source | CHO cells |
| Biological Activity | Determined by its ability to induce cell proliferation in T-cell enriched PBMCs. The expected ED50 is ≤0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg. |
| Purity Confirmation | > 98% by SDS-PAGE & HPLC analyses |
| Length [aa] | 506 |
| Molecular Weight | 75 kDa |
| Species Reactivity | Human, Mouse |
| Formulation | lyophilized |
| Protein Sequence | p35 Subunit: RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSA p40 Subunit: MWELEKDVYV VEVDW |
| Synonyms | NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF |
| Uniprot ID | p35: P43431; p40: P43432 |
| Protein RefSeq | p35: NP_001152896.1; p40: NP_001290173.1 |
| mRNA RefSeq | p35: NM_001159424.2; p40: NM_001303244.1 |