Description / human IL-12 protein
IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue type mast cells. Among its biological activities IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells and induces the development of IFN-gamma producing Th1 cells. Recombinant Human IL-12 is a 75.0 kDa heterodimeric glycoprotein consisting of disulfide-linked 35 kDa (p35) and 40 kDa (p40) subunits (503 total amino acid residues).
More Information
| Size | 2 µg |
|---|---|
| Source | CHO cells |
| Biological Activity | Assay #1: The ED50 as determined by the induction of IFN-γ from NK cells co-stimulated with IL-18 is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg. Assay #2: Determined by its ability to increase IFN-γ production by anti-TCR mAb- |
| Purity Confirmation | > 98% by SDS-PAGE & HPLC analyses |
| Length [aa] | 503 |
| Molecular Weight | 75 kDa |
| Species Reactivity | Monkley, Mouse, Human, Leech |
| Formulation | lyophilized |
| Protein Sequence | p35 Subunit: RNLPVA TPDPGMFPCL HHSQNLLRAV SNMLQKARQT LEFYPCTSEE IDHEDITKDK TSTVEACLPL ELTKNESCLN SRETSFITNG SCLASRKTSF MMALCLSSIY EDLKMYQVEF KTMNAKLLMD PKRQIFLDQN MLAVIDELMQ ALNFNSETVP QKSSLEEPDF YKTKIKLCIL LHAFRIRAVT IDRVMSYLNA S p40 Subunit: IWELKK DVYV |
| Synonyms | Interleukin-12, NKSF, CTL Maturation Factor (TCMF), Cytotoxic Lymphocyte Maturation Factor (CLMF), TSF |
| Uniprot ID | p35: P29459; p40: P29460 |
| Protein RefSeq | p35: NP_000873.2; p40: NP_002178.2 |
| mRNA RefSeq | p35: NM_000882.4; p40: NM_002187.3 |