Description / anti-mouse IL-10 receptor alpha monoclonal antibody
Human and mouse IL-10 receptors are structurally related to the IFN-gamma receptor. These receptors are members of the class II subgroup of the cytokine receptor superfamily. The deduced amino acid sequence of human IL-10 R is approximately 60% identical to mouse IL-10 R. Although human IL-10 has cross-species activities and is active on mouse cells, mouse IL-10 is species-specific in its actions and does not bind to the human IL-10 receptor. The human IL-10 R gene has been mapped to chromosome 11q23.3. Recombinant IL-10 soluble receptor, consisting of the extracellular domain of IL-10 R, binds IL-10 with high affinity in solution and is a potent IL-10 antagonist.
More Information
| Size | 100 µg |
|---|---|
| Source | Hamster |
| Isotype | IgG |
| Clone Nr. | (#9E12) |
| Species Reactivity | Mouse |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml. |
| Stability and Storage | Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity. |
| Preparation | This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with purified mouse recombinant protein of IL-10Rα extracellular domain. The IgG fraction of the culture supernatant was purified by Protein A/G affini |
| Antigen | Mouse recombinant EC domain of IL-10R |
| Application | WB, IHC (P) |
| Synonyms | Il10ra; Il10r; CDw210; CDw210a; mIL-10R; AW553859 |
| Uniprot ID | Q61727 |
| Protein RefSeq | NP_032374.1 |
| mRNA RefSeq | NM_008348.2 |