Description / human IL-10 protein
Interleukin-10 (IL-10), originally known as Cytokine Synthesis Inhibitory Factor (CSIF), is an 18.5 kDa protein that shares over 80% homology with the Epstein-Barr virus protein BCRFI. The reported biological activities of IL-10, which may be inter-related, include inhibition of macrophage mediated cytokine synthesis, suppression of the delayed type hyper-sensitivity response, and stimulation of the TH2 cell response which results in elevated antibody production.
More Information
| Size | 1000 µg |
|---|---|
| Source | E. coli |
| Biological Activity | Determined by the dose-dependent co-stimulation (with human IL-4) of MC/9 cells. The ED50 was found to be ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 105 units/mg. |
| Purity Confirmation | > 98% by SDS-PAGE & HPLC analyses |
| Length [aa] | 161 |
| Molecular Weight | 18.6 kDa |
| Species Reactivity | Monkey, Mouse, Human, Bacteria, Virus |
| Formulation | lyophilized |
| Buffer | 5mM Sodium Phosphate, pH 7.2 |
| Protein Sequence | MSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM KDQLDNLLLK ESLLEDFKGY LGCQALSEMI QFYLEEVMPQ AENQDPDIKA HVNSLGENLK TLRLRLRRCH RFLPCENKSK AVEQVKNAFN KLQEKGIYKA MSEFDIFINY IEAYMTMKIR N |
| Reconstitution | Centrifuge the vial prior to opening. Reconstitute in 5mM Sodium Phosphate, pH 7.2 to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a bu |
| Stability and Storage | The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 12 months at -20°C to -80°C. |
| Synonyms | IL10; CSIF; TGIF; GVHDS; IL-10; IL10A |
| Uniprot ID | P22301 |
| Protein RefSeq | NP_000563.1 |
| mRNA RefSeq | NM_000572 |