Description / anti-human IL-1 receptor antagonist monoclonal antibody
IL-1 receptor antagonist (IL-1ra) was originally isolated from the urine of patients with monocytic leukemia and has also been purified from adherent monocytes. The protein shows 26% amino acid homology to IL-1 beta and 19% homology to IL-1 alpha. It will compete with either factor for receptor binding, but does not interact with either one. Human IL-1ra will bind to both types of IL-1 receptor (I and II) on human cells. In mouse, IL-1RII does not bind IL-1ra.
More Information
| Size | 100 µg |
|---|---|
| Source | Rat |
| Isotype | IgG2 |
| Biological Activity | WB: 1:500-2000 |
| Clone Nr. | (#2V23) |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml. |
| Stability and Storage | Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity. |
| Preparation | This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat) immunized with human recombinant protein of IL-1ra. |
| Antigen | human recombinant protein of IL-1ra |
| Application | WB |
| Synonyms | IL1RN; DIRA; IRAP; IL1F3; IL1RA; MVCD4; IL-1RN; IL-1ra; IL-1ra3; ICIL-1RA |
| Uniprot ID | P18510 |
| Protein RefSeq | NP_776214.1 |
| mRNA RefSeq | NM_173842.2 |