Description / anti-mouse IGF-BP5 monoclonal antibody
IGFBP5 is expressed by fibroblasts, myoblasts and osteoblasts, making it the predominant IGFBP found in bone extracts. IGFBP5 has a strong affinity for hydroxyapatite, allowing it to bind to bone cells. When bound to extracelluar matrix, IGFBP5 is protected from proteolysis and potentiates IGF activity, but when it is soluble, IGFBP5 is cleaved to a biologically inactive 21 kDa fragment. Mouse, human and rat IGFBP5 share 97% identity.
More Information
| Size | 100 µg |
|---|---|
| Source | Rat |
| Isotype | IgG2 |
| Clone Nr. | (#12C23) |
| Species Reactivity | Mouse |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml. |
| Stability and Storage | Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity. |
| Preparation | This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with mouse recombinant protein of IGFBP-5. |
| Antigen | recombinant mouse protein of IGFBP-5 |
| Application | WB |
| Synonyms | Igfbp5; IGFBP-5; AI256729; AW208790; IGFBP-5P |
| Uniprot ID | Q07079 |
| Protein RefSeq | NP_034648.2 |
| mRNA RefSeq | NM_010518.2 |