Description / human IFN gamma protein
Interferon-gamma (IFN-gamma) is a lymphoid factor which possesses potent anti-viral activity. It has also been shown to stimulate macrophages and NK cells. Recombinant Human IFN-gamma is a 16.7 kDa protein containing 143 amino acid residues.
More Information
| Size | 1000 µg |
|---|---|
| Source | E. coli |
| Biological Activity | Assay #1: Determined by its ability to induce apoptosis in HeLa cells. The expected ED50 for this effect is 5.0-10.0 ng/ml. Assay #2: The ED50 was determined by a cytotoxicity assay using HT-29 cells is ≤ 0.05 ng/ml (> 2 x 107 units/mg). |
| Purity Confirmation | > 98% by SDS-PAGE & HPLC analyses |
| Length [aa] | 144 |
| Molecular Weight | 16.8 kDa |
| Species Reactivity | Monkey, Human, Mouse, Leech, Pig, |
| Formulation | lyophilized |
| Buffer | 10mM Sodium Phosphate, pH 7.4 |
| Protein Sequence | MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ |
| Reconstitution | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier p |
| Stability and Storage | The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 12 months at -20°C to -80°C. |
| Synonyms | IFNG; IFG; IFI |
| Uniprot ID | P01579 |
| Protein RefSeq | NP_000610.2 |
| mRNA RefSeq | NM_000619 |