Description / human IFN alpha 2b protein
Proteins of this family play an important role in inducing non-specific resistance against a broad range of viral infections. They also affect cell proliferation and modulate immune responses. Produced by peripheral blood leukocytes and lymphoblastoid cells. IFN alpha is an acid stable molecule that signals through IFN alpha/beta receptor (IFNα/βR or IFNAR), which is also used by IFN beta. Both IFNs have similar anti-viral activity and regulate expression of MHC class I antigens. IFN alpha contains four highly conserved cysteine residues which form two disulfide bonds, one of which is necessary for biological activity. The difference between IFNα subtype 2a and 2b is in the amino acid at position 23. While the subtype 2a has a lysine at position 23, the subtype 2b has an arginine. Recombinant Human IFN alpha 2b is a 19.4 kDa protein containing 166 amino acid residues.
More Information
| Size | 1000 µg |
|---|---|
| Source | E. coli |
| Biological Activity | The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 260,000,000 IU/ mg. |
| N Terminal Sequence | MCDLPQTHSL GSRRTLMLLA QMRRISLFSC LKDRHDFGFP QEEFGNQFQK AETIPVLHEM IQQIFNLFST KDSSAAWDET LLDKFYTELY QQLNDLEACV IQGVGVTETP LMKEDSILAV RKYFQRITLY LKEKKYSPCA WEVVRAEIMR SFSLSTNLQE SLRSKE |
| Purity Confirmation | ≥ 98% by SDS-PAGE gel and HPLC analyses. |
| Length [aa] | 166 |
| Molecular Weight | 19.4 kDa |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Reconstitution | It is recommended to reconstitute the lyophilized IFN-alpha 2b in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability and Storage | Lyophilized IFN although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN alpha 2b should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to |
| Synonyms | IFN alpha 2b, IFNA, INFA2, MGC125764, MGC125765. |
| Uniprot ID | P01563 |
| Protein RefSeq | NP_000596-2 |
| mRNA RefSeq | NM_000605-3 |