Description / human IFN alpha 2b protein
IFN-alpha is produced by macrophages and has antiviral activities. IFN stimulates the production of two enzymes: protein kinase and an oligoadenylate synthetase. IFN-a 2b Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 166 amino acids and having a molecular mass of 19400 Dalton. The difference between IFNA2A and IFNA2B is in the amino acid present at position 23. IFN-alpha 2a has a lysine at that position 23 while IFN-alpha 2b has arginine. The IFN-alpha 2b gene was obtained from human leukocytes.
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| Size | 20 µg |
|---|---|
| Source | E. coli |
| Biological Activity | The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 260,000,000 IU/ mg. |
| N Terminal Sequence | MCDLPQTHSL GSRRTLMLLA QMRRISLFSC LKDRHDFGFP QEEFGNQFQK AETIPVLHEM IQQIFNLFST KDSSAAWDET LLDKFYTELY QQLNDLEACV IQGVGVTETP LMKEDSILAV RKYFQRITLY LKEKKYSPCA WEVVRAEIMR SFSLSTNLQE SLRSKE |
| Purity Confirmation | ≥ 98% by SDS-PAGE gel and HPLC analyses. |
| Length [aa] | 166 |
| Molecular Weight | 19.4 kDa |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Reconstitution | It is recommended to reconstitute the lyophilized IFN-alpha 2b in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability and Storage | Lyophilized IFN although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN alpha 2b should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to |
| Synonyms | IFN alpha 2b, IFNA, INFA2, MGC125764, MGC125765. |
| Uniprot ID | P01563 |
| Protein RefSeq | NP_000596-2 |
| mRNA RefSeq | NM_000605-3 |