Description / anti-human ICAM-1 (CD54) monoclonal antibody
ICAMs are members of the Ig superfamily of calcium-independent transmembrane glycoproteins. ICAM-1 is a ligand for lymphocyte function-associated (LFA) and Mac-1 integrins and the major human rhinovirus receptor. The primary function of ICAM-1 is to provide adhesion between endothelial cells and leukocytes after stress or injury. The human ICAM-1 gene codes for a 505 amino acid transmembrane glycoprotein containing a 29 amino acid cytoplasmic domain, a 23 amino acid transmembrane domain, and a 453 amino acid extracellular domain. Recombinant human ICAM-1 is a 49.5 kDa glycoprotein comprising the extracellular domain (453 amino acid residues) of ICAM-1. Monomeric glycosylated ICAM-1 migrates at an apparent molecular weight of approximately 72.0-80.0 kDa by SDS-PAGE analysis under reducing conditions.
More Information
| Size | 50 µg |
|---|---|
| Source | Mouse |
| Isotype | IgG1 |
| Biological Activity | WB: 1:100-1000; IHC (Formalin/Paraffin): 1:25-50; ELISA: Yes |
| Clone Nr. | (#7D22) |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml. |
| Stability and Storage | Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity. |
| Preparation | This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with human ICAM-1 N-terminal recombinant protein). The IgG1 fraction of culture supernatant was purified by Protein G affinity chromatography. |
| Antigen | recombinant human ICAM1 N-terminal protein |
| Application | ELISA, WB, IHC (Formalin/P) |
| Synonyms | ICAM1; BB2; CD54; P3.58 |
| Uniprot ID | P05362 |
| Protein RefSeq | NP_000192.2 |
| mRNA RefSeq | NM_000201 |